ࡱ> =?<a R*'bjbj 20hGbhGb   8<- IIIIInnn$i"%h" !nnnnn" II%C nnnnIInnnnV\HI %fn Y 0  %n%%,nnnnnnnn" " nnnn nnnn%nnnnnnnnn Q k:  x'`ˆ}vvAcid protease, ACP ՋBRvffN _ϑl100T/48S l ack_Km[KNMR b2-3*Ng]_'Yv7h,gZPKm[0 Km[aIN ACP/fNy(Wx'`sX NPSˆ}v(4l㉄vv0v;N(uNR|Su0dUR 0kvoS0gRon0ql 0reI{0 Km[St x'`agN N ACPPSjˆ}v4l㉧Nuj(lx(Wx'`agN N j(lx؏SxxSTirub݄݄(W680nm gyr_8T6e\ ǏKm[vQ8TIQ^XR eg{ACP;m'`0 YNhVT7hT 4ltm0xRdbhV0S_ymg0SRIQIQ^/vhN0_ϑstkrv/96T[g00.5 mL EP{T4l0 ՋBR~bTMn mSON2mL1/e 4!OX[0 mSON1mL1/e 4!OX[0 ՋBRNvM6R4N(uMR cmSONmSON4l=90L 20L 21mL vkOM6R sMs(u YQs}vrn}rlmR N(u0 ՋBRN|BR1t 4!OX[04N(uMRR4mL4ln0 ՋBR N|BR1t 4!IQOX[04N(uMRReQ10mLՋBRN l4ltm-NxRdbn0S(Wpog NvNB\O la‰[ MQ4lRhQ萸S N,Rp15-30R ՋBR:NǏqTՋBR EQRmSTNQs|ir Nnir Nq_TO(u 0 ՋBRV|BR1t 4!OX[04N(uMRReQ20mL4ln0 ՋBRNmSO4mL1t 4!OX[0 hQTmSO1mL1/e 0.25mol/mLhQj(lxnm 4!OX[0 |vmcS ՋBRNvM6RՋBRv~bTM6R0 20~~ cgq~~(ϑg ՋBRNSOy(mL):N15~10vkO^yS~0.1g~~ ReQ1mLՋBRN QtmSFm 8000g 4!y_10min S Nn sS|vm0 @nbW{QmvcKm[0 ~̃0w̃ cgq~ހpeϑ104*N ՋBRNSOymL :N500~10001vkO^500N~ހReQ1mLՋBRN QtmXl4xx~ހRs300w X3y 7y ;`e3min 6qT8000g 4! y_10min S NnnNQ N_Km0 Km[d\O 1.RIQIQ^/vhNp30min l0R680 nm 4l0 2.ՋBRN0ՋBR NTՋBRVnN30!4ltmO)n30min0 3. [gq{S0.5 mL EP{ ReQ20L|vm 40LՋBRN mSTnN30!4ltmO)n10minReQ40LՋBR N mST8000g 4!y_10minS40L Nnm ReQevEP{ QReQ200LՋBRV 40LՋBRN mSTnN30!4ltmO)n20min S200LN_ϑstkrv/96T[g 680nmKm[IQ8T6e :NA[gq{0 4. Km[{S0.5 mL EP{ ReQ20L|vm 40LՋBR N mSTnN30!4ltmO)n10minReQ40LՋBRN mST8000g 4!y_10minS4B z * >   ǰp_p_p_pV_V_VG;h_CJOJQJaJh_CJOJQJ^JaJo(h_CJaJo( h_CJOJQJ\^JaJo($h_5CJOJPJQJ^JaJo(h_CJOJQJ\aJo(h_5CJOJQJaJo(h_5CJOJQJaJo(-h_B*CJOJPJQJ\^JaJo(ph$h_CJOJPJQJ\^JaJo($h_5CJOJPJQJ^JaJo($h_CJOJPJQJ\^JaJo(B z | * , >  ` b p B & F$a$ > B L P \ ^ ` l " ` j 6 D ` b p ƻƤƑ~uhu`uh_CJaJh_CJOJQJaJo(h_CJaJo($h_5CJOJPJQJ^JaJo($h_CJOJPJQJ\^JaJo(h_CJOJQJ\aJh_CJOJaJh_CJOJaJo( h_CJOJQJ\^JaJo(h_CJOJQJ^JaJo(h_CJOJQJaJh_CJOJQJaJo(%",6>\z|,.028BXZh".檵梵ُ~m~`~h_CJOJQJ\aJ h_5CJOJQJ^JaJo( h_CJOJQJ\^JaJo($h_5CJOJPJQJ^JaJo(h_CJaJh_CJOJaJo(h_CJOJaJh_CJH*OJQJaJh_CJOJQJaJo(h_CJOJQJaJo(h_CJOJQJaJh_CJOJQJaJo(%B\Z\hT !!!!L"##(##,$>$$r%& & F & F.2fjxXZ*.:>rv&*6:nr > B ᢏ$h_5CJOJPJQJ^JaJo($h_CJOJPJQJ\^JaJo(U h_5CJOJQJ^JaJo(h_CJOJQJaJo(h_CJOJQJaJ h_CJOJQJ\^JaJo(h_CJOJQJ\aJ60L Nnm ReQevEP{ QReQ200LՋBRV 40LՋBRN mSTnN30!4ltmO)n20min S200LN_ϑstkrv/96T[g N680nmKm[IQ8T6e :NAKm[{0laNzz}v{ N T HQRՋBR N TRՋBRN 5. zz}v{S0.5 mL EP{ ReQ40L4l 200LՋBRV 40LՋBRN mSTnN30!4ltmO)n20min S200LN_ϑstkrv/96T[g N680nmKm[IQ8T6e :NAzz}v{0 6. hQ{S0.5 mL EP{ ReQ40LhQT 200LՋBRV 40LՋBRN mSTnN30!4ltmO)n20min S200LN_ϑstkrv/96T[g N680nmKm[IQ8T6e :NAhQ{0 ACP;m'`{lQ_ 1. cgq7h,gˆ}vSm^{ ACP;m'`USMO[IN30!kkKQˆ}vkR4l㉧Nu1nmolj(lx:N1*Nv;mUSMO0 ACP;m'`nmol/min /mg prot = ChQTAKm[{ A[gq{ AhQ{ Azz}v{ VS;`(CprV1)T= 125AKm[{ A[gq{ AhQ{ Azz}v{ Cpr 2 cgq7h,g(ϑ{ ACP;m'`USMO[IN30!kKQ7hTkRPS4l㉧Nu1 nmolj(lx:N1*Nv;mUSMO0 ACP;m'`nmol/min /g͑ =ChQTAKm[{ A[gq{ AhQ{ Azz}v{ VS;`WV1V2 T= 125AKm[{ A[gq{ AhQ{ Azz}v{ W 3. cgqmSOSOy{ ACP;m'`USMO[IN30!kkGS7hTkRPS4l㉧Nu1nmolj(lx:N1*Nv;mUSMO0 ACP;m'`nmol/min/mL =ChQTAKm[{ A[gq{ AhQ{ Azz}v{ VS;`V1T= 125AKm[{ A[gq{ AhQ{ Azz}v{ cgq~ހpeϑ{ ACP;m'`USMO[IN30!k104*N~ހkRPS4l㉧Nu1nmolj(lx:N1*Nv;mUSMO0 ACP;m'`nmol/min /104 cell =ChQTAKm[{ A[gq{ AhQ{ Azz}v{ VS;`~ހpeϑV1V2 T= 125AKm[{ A[gq{ AhQ{ Azz}v{ ~ހpeϑ ChQT0.25  mol/mLhQj(lxnmVS;`vOS^;`SOy 0.1mLCpr|vmˆ}v(Sm^mg/mL V1ReQS^SO|-N|vmSOymL 0.02 mLV2cSm;`SOymL 1mLTPSS^emin 10minW7hT(ϑg 0 laNy 1.ՋBRN cd\Oc:yM6R (uY\MY\ Qs}vrn}rlmR N(u0 2.4N(uMRM6RvՋBRMn}YT3)YQO(u[k0     ! !!!!*!!@!B!Z!^!|!!!!!!!!!!!!!" " "",".":"H"L"P"X"h"n"r"""""""""#(#,#<#>#@#B#D#H#`#b#n#|#######ʽֽֽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽʽh_CJOJQJaJo(h_CJOJQJaJh_CJOJQJaJh_CJOJQJaJo(h_5CJOJQJ\aJo(J####### $$*$B$R$T$V$X$^$`$f$z$|$$$$$$$$$$$$%%%%.%2%F%J%h%r%%%&"&0&6&t&v&&&&&&& ' ''綩 h_CJOJQJ\^JaJo(h_5CJOJQJaJh_5CJOJQJaJo(h_CJaJo(h_CJH*OJQJaJh_CJOJQJaJh_CJOJQJaJo(h_CJOJQJaJ9&&&& ''''''' '$'&'('*''''''' '"'('*' h_CJOJQJ\^JaJo(h_jh_U 0182P. A!"#$%S  s666666666vvvvvvvvv666446>6666666666666666666666666666666666666666666666666hH66666666666666666666666666666666666666666666666666666666666666666668 0@P`p6(88 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p8XV~ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ OJPJQJ_HmH nHsH tHJ`J cke $1$a$ CJKH_HaJmH nHsH tH$A $ ؞k=W[SONiN nfhP hXD<YDa$$@&CJ OJQJaJ 5\NbN 0u w'a$$G$&dP 9r CJaJ< r< 0ua$$G$ 9r CJaJPK![Content_Types].xmlN0EH-J@%ǎǢ|ș$زULTB l,3;rØJB+$G]7O٭V&Ŝ]6ҨN=Ina7qb&>!"h [+Lxz)WVOg%Ţ|8Mă#/8'=QU*C`CW{D6/[YL%+n+{\osM/~iBcAX/=x E'5&\h4da,yE\})laR^*zyV-WoWZN]:mV=tCWrb*IVURU|nc`|*^PK! ѐ'theme/theme/_rels/themeManager.xml.relsM 0wooӺ&݈Э5 6?$Q ,.aic21h:qm@RN;d`o7gK(M&$R(.1r'JЊT8V"AȻHu}|$b{P8g/]QAsم(#L[PK-![Content_Types].xmlPK-!֧6 0_rels/.relsPK-!kytheme/theme/themeManager.xmlPK-!ftheme/theme/theme1.xmlPK-! ѐ' theme/theme/_rels/themeManager.xml.relsPK] 0  . #'*' B&*'  Bs>@0(  B S  ?kke*e*|sI|sIh^h`o(0H\^H`\)\^`\.\^`\.4\^4`\) \^ `\.| \^| `\. \^ `\)\^`\.hh^h`QJ.^`.pp^p`.@ @ ^@ `.^`.^`.^`.^`.PP^P`.h^h`o(0H\^H`\)\^`\.\^`\.4\^4`\) \^ `\.| \^| `\. \^ `\)\^`\.k|sIe*EK]xm?g'  ?!0<$u%!':' 0b-:b?.^E$IK[M(OWz\_ gi0]jV%n"ptp[r}u+voy,{b}k$tm5q3ho&HPjooeCcKid0Fih~2B | :CMuY @   UnknownG.[x Times New Roman5Symbol3. .[x Arial;= |8ўSOSimHei;([SOSimSun7..{$ Calibri7$BCambriaA$BCambria Math Qho;G$GQLd'llZ&!),.:;?]}    & 6"0000 0 0 00000 =@\]^([{  0 0 00000;[ KQ) ?'*2!xx x'`ˆ}vv;m'`Km[ՋBRvffNyjm Administrator   Oh+'0 , L X d p| ԵøԲⶨԼ˵yjmNormalAdministrator2Microsoft Office Word@@N҃@"`t@wfl ՜.+,D՜.+,|8   (0yjm  ` ?GKSOProductBuildVer2052-9.1.0.5041  !"#$%&'()*+-./012356789:;>Root Entry FP퍔f@1Table%WordDocument20SummaryInformation(,DocumentSummaryInformation84CompObjn  FMicrosoft Word 97-2003 ĵ MSWordDocWord.Document.89q